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ATP1A1 Recombinant antibody

ATP1A1 Recombinant Antibody for IF, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

240160B5

Cat no : 83191-5-RR

Synonyms

ATP1A1, Sodium pump subunit alpha 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ATP1A1 Recombinant antibody (83191-5-RR)

    IF Staining of A431 using 83191-5-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATP1A1 antibody (83191-5-RR, Clone: 240160B5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ATP1A1 Recombinant antibody (83191-5-RR)

    IF Staining of A431 using 83191-5-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATP1A1 antibody (83191-5-RR, Clone: 240160B5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of MCF-7 cells using ATP1A1 Recombinant antibody (83191-5-RR)

    FC experiment of MCF-7 using 83191-5-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug ATP1A1 Recombinant antibody (83191-5-RR, Clone:240160B5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inA431 cells
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:500-1:2000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83191-5-RR is ATP1A1 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ATP1A1 fusion protein Ag5763
Full Name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Calculated Molecular Weight 113 kDa
GenBank Accession NumberBC050359
Gene Symbol ATP1A1
Gene ID (NCBI) 476
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein.

Protocols

Product Specific Protocols
IF protocol for ATP1A1 antibody 83191-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols