ATP1A1 Recombinant antibody, PBS Only

ATP1A1 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240160B5

Cat no : 83191-5-PBS

Synonyms

Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13, Atpa 1, ATP1A 1

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Validation Data Gallery

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  IF Staining of A431 using 83191-5-RR (same clone as 83191-5-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATP1A1 antibody (83191-5-RR, Clone: 240160B5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83191-5-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 83191-5-RR (same clone as 83191-5-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using ATP1A1 antibody (83191-5-RR, Clone: 240160B5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83191-5-PBS in a different storage buffer formulation.

• 

  FC experiment of HEK-293 using 83191-5-RR (same clone as 83191-5-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.2 ug ATP1A1 Recombinant antibody (83191-5-RR, Clone:240160B5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83191-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83191-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83191-5-RR against Human ATP1A1 were performed. The affinity constant is 87.7 pM.

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Product Information

The immunogen of 83191-5-PBS is ATP1A1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ATP1A1 fusion protein Ag5763
• Full Name: ATPase, Na+/K+ transporting, alpha 1 polypeptide
• Calculated Molecular Weight: 113 kDa
• GenBank Accession Number: BC050359
• Gene Symbol: ATP1A1
• Gene ID (NCBI): 476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.