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Acetyl-Histone H2B (Lys5) Recombinant antibody

Acetyl-Histone H2B (Lys5) Recombinant Antibody for WB, IF, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230539G10

Cat no : 83171-4-RR

Synonyms

GL105, H2B, H2B.1, H2B/q, H2BFQ, HIST2H2BE, histone cluster 2, H2be, Histone H2B GL105, Histone H2B type 2 E, Histone H2B.q

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using Acetyl-Histone H2B (Lys5) Recombinant antibody (83171-4-RR)

    WB analysis of NIH/3T3 using 83171-4-RR

    Trichostatin A treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83171-4-RR (Acetyl-Histone H2B (Lys5) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Acetyl-Histone H2B (Lys5) Recombinant antibody (83171-4-RR)

    IF Staining of HeLa using 83171-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2B (Lys5) antibody (83171-4-RR, Clone: 230539G10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Acetyl-Histone H2B (Lys5) Recombinant antibody (83171-4-RR)

    IF Staining of HeLa using 83171-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2B (Lys5) antibody (83171-4-RR, Clone: 230539G10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using Acetyl-Histone H2B (Lys5) Recombinant antibody (83171-4-RR)

    FC experiment of HeLa using 83171-4-RR

    1x10^6 TSA treated HeLa cells were intracellularly stained with 0.25 ug Acetyl-Histone H2b (Lys5) Recombinant Antibody (83171-4-RR, Clone:230539G10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Tested Applications

Positive WB detected inTrichostatin A treated NIH/3T3 cells
Positive IF detected inHeLa cells
Positive FC detected inTSA treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83171-4-RR targets Acetyl-Histone H2B (Lys5) in WB, IF, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name histone cluster 2, H2be
Calculated Molecular Weight 14 kDa
Observed Molecular Weight15 kDa
GenBank Accession NumberBC005827
Gene Symbol HIST2H2BE
Gene ID (NCBI) 8349
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Acetyl-Histone H2B (Lys5) antibody 83171-4-RRDownload protocol
IF protocol for Acetyl-Histone H2B (Lys5) antibody 83171-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols