Immunofluorescent analysis of (4% PFA) fixed Hela cells using CoraLite® Plus 488 Acetyl-Histone H2B (Lys5) antibody (CL488-83171-4, Clone: 230539G10 ) at dilution of 1:200.
Immunofluorescent analysis of (4% PFA) fixed Hela cells using CoraLite® Plus 488 Acetyl-Histone H2B (Lys5) antibody (CL488-83171-4, Clone: 230539G10 ) at dilution of 1:200.
FC experiment of HeLa using CL488-83171-4
1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Acetyl-Histone H2b (Lys5) Recombinant Antibody (CL488-83171-4, Clone:230539G10)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Acetyl-Histone H2b (Lys5) Recombinant Antibody (CL488-83171-4, Clone:230539G10)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Acetyl-Histone H2b (Lys5) Recombinant Antibody (CL488-83171-4, Clone:230539G10)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
IF/ICC Figures
IF Staining of Hela using CL488-83171-4
Immunofluorescent analysis of (4% PFA) fixed Hela cells using CoraLite® Plus 488 Acetyl-Histone H2B (Lys5) antibody (CL488-83171-4, Clone: 230539G10 ) at dilution of 1:200.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Acetyl-Histone H2B (Lys5) Recombinant antibody
Catalog Number
CL488-83171-4
Citations
-
Dilutions
IF/ICC : 1:50-1:500 FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Applications
IF/ICC, FC (Intra)
Reactivity
human, mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
