CoraLite® Plus 488-conjugated Acetyl-Histone H2B (Lys5) Recombinant antibody

Acetyl-Histone H2B (Lys5) Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230539G10

Cat no : CL488-83171-4

Synonyms

HIST2H2BE, Histone H2B, H2BC21, H2B/q, H2B.1

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Validation Data Gallery

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  IF Staining of Hela using CL488-83171-4

  Immunofluorescent analysis of (4% PFA) fixed Hela cells using CoraLite® Plus 488 Acetyl-Histone H2B (Lys5) antibody (CL488-83171-4, Clone: 230539G10 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-83171-4

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Acetyl-Histone H2b (Lys5) Recombinant Antibody (CL488-83171-4, Clone:230539G10)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

• Positive IF/ICC detected in: Hela cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83171-4 targets Acetyl-Histone H2B (Lys5) in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: histone cluster 2, H2be
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC005827
• Gene Symbol: Histone H2B
• Gene ID (NCBI): 8349
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.