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  • Western Blot (WB) analysis of HEK-293 cells using MTAP Recombinant antibody (83005-1-RR)

    WB analysis of HEK-293 using 83005-1-RR (same clone as 83005-1-PBS)

    WB result of MTAP antibody (83005-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTAP transfected HEK-293 cells. This data was developed using the same antibody clone with 83005-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using MTAP Recombinant antibody (83005-1-RR)

    WB analysis using 83005-1-RR (same clone as 83005-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83005-1-RR (MTAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83005-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83005-1-RR

    Affinity and Kinetic Characterization of 83005-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83005-1-RR against Human MTAP were performed. The affinity constant is 0.782 nM.

  • Featured Product
  • KD/KO Validated

MTAP Recombinant antibody, PBS Only

MTAP Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 83005-1-PBS
Clone No.240030A3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

240030A3, 5'-methylthioadenosine phosphorylase, c86fus, EC:2.4.2.28, MSAP

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Product Information

83005-1-PBS targets MTAP in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MTAP fusion protein Ag2051 Predict reactive species
Full Name methylthioadenosine phosphorylase
Calculated Molecular Weight 283 aa, 31 kDa
Observed Molecular Weight31 kDa
GenBank Accession NumberBC018625
Gene Symbol MTAP
Gene ID (NCBI) 4507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13126
Storage Buffer PBS only, pH 7.3.
Storage ConditionsStore at -80°C.

Background Information

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.