MTAP Recombinant antibody

MTAP Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240030A3

Cat no : 83005-1-RR

Synonyms

c86fus, MSAP, MTA phosphorylase, MTAP, MTAPase

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Validation Data Gallery

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  WB analysis using 83005-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83005-1-RR (MTAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83005-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83005-1-RR against Human MTAP were performed. The affinity constant is 0.782 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, HCT 116 cells, HT-29 cells, NIH/3T3 cells, C6 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83005-1-RR targets MTAP in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MTAP fusion protein Ag2051
• Full Name: methylthioadenosine phosphorylase
• Calculated Molecular Weight: 283 aa, 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC018625
• Gene Symbol: MTAP
• Gene ID (NCBI): 4507
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MTAP is a 5-deoxy-5-methylthioadenosine (MTA) phosphorylase, converting MTA to salvageable intermediates including adenine and 5-methylthioribose-1-phosphate. MTAP is abundant in normal cells, but it is frequently found to be deleted in a variety of cancers. Its deficiency is common in cancer cell lines as well as in primary leukemia, lung cancer, melanoma, bladder cancer, gliomas and breast cancer.