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YY1 Recombinant antibody

YY1 Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2K12

Cat no : 82712-5-RR

Synonyms

NF-E1, INO80S, INO 80S, Delta transcription factor, DELTA

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using YY1 Recombinant antibody (82712-5-RR)

    IF Staining of HepG2 using 82712-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (82712-5-RR, Clone: 2K12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using YY1 Recombinant antibody (82712-5-RR)

    IF Staining of HepG2 using 82712-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (82712-5-RR, Clone: 2K12 ) at dilution of 1:700 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 82712-5-RR

    Affinity and Kinetic Characterization of 82712-5-RR

    Biolayer interferometry (BLl) kinetic assays of 82712-5-RR against Human YY1 were performed. The affinity constant is 0.412 pM.

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:350-1:1400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82712-5-RR targets YY1 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen YY1 fusion protein Ag17792
Full Name YY1 transcription factor
Calculated Molecular Weight 414 aa, 45 kDa
GenBank Accession NumberBC037308
Gene Symbol YY1
Gene ID (NCBI) 7528
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.

Protocols

Product Specific Protocols
IF protocol for YY1 antibody 82712-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols