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MAVS; VISA Recombinant antibody

MAVS; VISA Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

7F14

Cat no : 81910-1-RR

Synonyms

MAVS, KIAA1271, IPS-1, IPS1, IPS 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAVS; VISA Recombinant antibody (81910-1-RR)

    WB analysis using 81910-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81910-1-RR (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using MAVS; VISA Recombinant antibody (81910-1-RR)

    IP experiment of HEK-293 using 81910-1-RR

    IP result of anti-MAVS; VISA (IP:81910-1-RR, 4ug; Detection:81910-1-RR 1:4000) with HEK-293 cells lysate 1480 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MAVS; VISA Recombinant antibody (81910-1-RR)

    IHC staining of human breast cancer using 81910-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81910-1-RR (MAVS; VISA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using MAVS; VISA Recombinant antibody (81910-1-RR)

    IHC staining of human breast cancer using 81910-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81910-1-RR (MAVS; VISA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MAVS; VISA Recombinant antibody (81910-1-RR)

    IF Staining of HeLa using 81910-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAVS; VISA antibody (81910-1-RR, Clone: 7F14 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using MAVS; VISA Recombinant antibody (81910-1-RR)

    IF Staining of HeLa using 81910-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAVS; VISA antibody (81910-1-RR, Clone: 7F14 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 81910-1-RR

    Affinity and Kinetic Characterization of 81910-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81910-1-RR against Human MAVS; VISA were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, A431 cells, A549 cells, MCF-7 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81910-1-RR targets MAVS; VISA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MAVS; VISA fusion protein Ag5655
Full Name mitochondrial antiviral signaling protein
Calculated Molecular Weight 57 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC044952
Gene Symbol MAVS
Gene ID (NCBI) 57506
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN-β promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.

Protocols

Product Specific Protocols
WB protocol for MAVS; VISA antibody 81910-1-RRDownload protocol
IHC protocol for MAVS; VISA antibody 81910-1-RRDownload protocol
IF protocol for MAVS; VISA antibody 81910-1-RRDownload protocol
IP protocol for MAVS; VISA antibody 81910-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols