CoraLite® Plus 488-conjugated MAVS; VISA Recombinant antibody

MAVS; VISA Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

7F14

Cat no : CL488-81910

Synonyms

MAVS, KIAA1271, IPS-1, IPS1, IPS 1

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Validation Data Gallery

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  IF Staining of HeLa using CL488-81910

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 MAVS; VISA antibody (CL488-81910, Clone: 7F14 ) at dilution of 1:100.

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  FC experiment of U2OS using CL488-81910

  1x10^6 U2OS cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated MAVS; VISA Recombinant antibody (CL488-81910, Clone:7F14)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-81910 targets MAVS; VISA in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MAVS; VISA fusion protein Ag5655
• Full Name: mitochondrial antiviral signaling protein
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC044952
• Gene Symbol: MAVS
• Gene ID (NCBI): 57506
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN-β promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.