MAVS; VISA Recombinant antibody, PBS Only

MAVS; VISA Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

7F14

Cat no : 81910-1-PBS

Synonyms

MAVS, KIAA1271, IPS-1, IPS1, IPS 1

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Validation Data Gallery

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  WB analysis using 81910-1-RR (same clone as 81910-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81910-1-RR (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 81910-1-RR (same clone as 81910-1-PBS)

  IP result of anti-MAVS; VISA (IP:81910-1-RR, 4ug; Detection:81910-1-RR 1:4000) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 81910-1-RR (same clone as 81910-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81910-1-RR (MAVS; VISA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 81910-1-RR (same clone as 81910-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 81910-1-RR (MAVS; VISA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAVS; VISA antibody (81910-1-RR, Clone: 7F14 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 81910-1-RR (same clone as 81910-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAVS; VISA antibody (81910-1-RR, Clone: 7F14 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81910-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81910-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 81910-1-RR against Human MAVS; VISA were performed. The affinity constant is below 1 pM.

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Product Information

81910-1-PBS targets MAVS; VISA in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MAVS; VISA fusion protein Ag5655
• Full Name: mitochondrial antiviral signaling protein
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC044952
• Gene Symbol: MAVS
• Gene ID (NCBI): 57506
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN-β promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.