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UBE2S Recombinant antibody

UBE2S Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

6D18

Cat no : 81854-1-RR

Synonyms

EPF5, EC:2.3.2.23, E2-EPF, E2EPF, E2 EPF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBE2S Recombinant antibody (81854-1-RR)

    WB analysis using 81854-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81854-1-RR (UBE2S antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using UBE2S Recombinant antibody (81854-1-RR)

    IHC staining of mouse testis using 81854-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 81854-1-RR (UBE2S antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using UBE2S Recombinant antibody (81854-1-RR)

    IHC staining of mouse testis using 81854-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 81854-1-RR (UBE2S antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • Immunofluorescence (IF) / fluorescent staining of NCCIT cells using UBE2S Recombinant antibody (81854-1-RR)

    IF Staining of NCCIT using 81854-1-RR

    Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using UBE2S antibody (81854-1-RR, Clone: 6D18 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 81854-1-RR

    Affinity and Kinetic Characterization of 81854-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81854-1-RR against Human UBE2S were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, HepG2 cells, K-562 cells, NIH/3T3 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNCCIT cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81854-1-RR targets UBE2S in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen UBE2S fusion protein Ag5264
Full Name ubiquitin-conjugating enzyme E2S
Calculated Molecular Weight 222 aa, 24 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC066948
Gene Symbol UBE2S
Gene ID (NCBI) 27338
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).

Protocols

Product Specific Protocols
WB protocol for UBE2S antibody 81854-1-RRDownload protocol
IHC protocol for UBE2S antibody 81854-1-RRDownload protocol
IF protocol for UBE2S antibody 81854-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols