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IGF2BP3 Recombinant antibody

IGF2BP3 Uni-rAbTM Recombinant Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1L11

Cat no : 81805-1-RR

Synonyms

IMP3, IMP 3, IGF-II mRNA-binding protein 3, IGF2BP 3, IGF2 mRNA-binding protein 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IGF2BP3 Recombinant antibody (81805-1-RR)

    WB analysis using 81805-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81805-1-RR (IGF2BP3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using IGF2BP3 Recombinant antibody (81805-1-RR)

    IP experiment of HeLa using 81805-1-RR

    IP result of anti-IGF2BP3 (IP:81805-1-RR, 4ug; Detection:81805-1-RR 1:20000) with HeLa cells lysate 1280 ug.

  • Immunoprecipitation (IP) experiment of HeLa cells using IGF2BP3 Recombinant antibody (81805-1-RR)

    IP experiment of HeLa using 81805-1-RR

    IP result of anti-IGF2BP3 (IP:81805-1-RR, 4ug; Detection:81805-1-RR 1:4000) with HeLa cells lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using IGF2BP3 Recombinant antibody (81805-1-RR)

    IHC staining of human pancreas cancer using 81805-1-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81805-1-RR (IGF2BP3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using IGF2BP3 Recombinant antibody (81805-1-RR)

    IHC staining of human pancreas cancer using 81805-1-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81805-1-RR (IGF2BP3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using IGF2BP3 Recombinant antibody (81805-1-RR)

    IHC staining of human pancreas cancer using 81805-1-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81805-1-RR (IGF2BP3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using IGF2BP3 Recombinant antibody (81805-1-RR)

    IHC staining of human pancreas cancer using 81805-1-RR

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81805-1-RR (IGF2BP3 antibody) at dilution of 1:400 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 81805-1-RR

    Affinity and Kinetic Characterization of 81805-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81805-1-RR against Human IGF2BP3 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells, mouse placenta tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

81805-1-RR targets IGF2BP3 in WB, IHC, IP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IGF2BP3 fusion protein Ag6226
Full Name insulin-like growth factor 2 mRNA binding protein 3
Calculated Molecular Weight 64 kDa
Observed Molecular Weight64 kDa
GenBank Accession NumberBC065269
Gene Symbol IGF2BP3
Gene ID (NCBI) 10643
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGF2BP3, also named IMP3, KOC1, and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma. Both isoforms (64kd and 22kd) of IGFBP3 can be recognized by this antibody. And IGFBP3 is nuclear and cytoplasm stains.

Protocols

Product Specific Protocols
WB protocol for IGF2BP3 antibody 81805-1-RRDownload protocol
IHC protocol for IGF2BP3 antibody 81805-1-RRDownload protocol
IP protocol for IGF2BP3 antibody 81805-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Sci Rep

Synchronous profiling of mRNA N6-methyladenosine modifications and mRNA expression in high-grade serous ovarian cancer: a pilot study

Authors - Li Yang
View Article