IGF2BP3 Recombinant antibody, PBS Only

IGF2BP3 Uni-rAb^TM Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1L11

Cat no : 81805-1-PBS

Synonyms

IMP3, IMP 3, IGF-II mRNA-binding protein 3, IGF2BP 3, IGF2 mRNA-binding protein 3

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Validation Data Gallery

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  WB analysis using 81805-1-RR (same clone as 81805-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81805-1-RR (IGF2BP3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81805-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 81805-1-RR (same clone as 81805-1-PBS)

  IP result of anti-IGF2BP3 (IP:81805-1-RR, 4ug; Detection:81805-1-RR 1:20000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 81805-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 81805-1-RR (same clone as 81805-1-PBS)

  IP result of anti-IGF2BP3 (IP:81805-1-RR, 4ug; Detection:81805-1-RR 1:4000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 81805-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 81805-1-RR (same clone as 81805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81805-1-RR (IGF2BP3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81805-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical squamous cancer using 81805-1-RR (same clone as 81805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 81805-1-RR (IGF2BP3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81805-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 81805-1-RR (same clone as 81805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81805-1-RR (IGF2BP3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81805-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 81805-1-RR (same clone as 81805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81805-1-RR (IGF2BP3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81805-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 81805-1-RR (same clone as 81805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81805-1-RR (IGF2BP3 antibody) at dilution of 1:400 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81805-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 81805-1-RR (same clone as 81805-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 81805-1-RR (IGF2BP3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81805-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81805-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81805-1-RR against Human IGF2BP3 were performed. The affinity constant is below 1 pM.

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Product Information

81805-1-PBS targets IGF2BP3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IGF2BP3 fusion protein Ag6226
• Full Name: insulin-like growth factor 2 mRNA binding protein 3
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC065269
• Gene Symbol: IGF2BP3
• Gene ID (NCBI): 10643
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IGF2BP3, also named IMP3, KOC1, and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma. Both isoforms (64kd and 22kd) of IGFBP3 can be recognized by this antibody. And IGFBP3 is nuclear and cytoplasm stains.