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  • Featured Product
  • KD/KO Validated

c-MYC Recombinant antibody

c-MYC Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B17

Cat no : 80845-1-RR

Synonyms

MYC, MRTL, Class E basic helix-loop-helix protein 39, c Myc, bHLHe39

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using c-MYC Recombinant antibody (80845-1-RR)

    WB analysis using 80845-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80845-1-RR (MYC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using c-MYC Recombinant antibody (80845-1-RR)

    WB analysis of A549 using 80845-1-RR

    WB result of MYC antibody (80845-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYC transfected A549 cells.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using c-MYC Recombinant antibody (80845-1-RR)

    IHC staining of mouse brain using 80845-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 80845-1-RR (c-MYC antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using c-MYC Recombinant antibody (80845-1-RR)

    IF Staining of HeLa using 80845-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MYC antibody (80845-1-RR, Clone: 2B17 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using c-MYC Recombinant antibody (80845-1-RR)

    IF Staining of HEK-293 using 80845-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MYC antibody (80845-1-RR, Clone: 2B17 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using c-MYC Recombinant antibody (80845-1-RR)

    IF Staining of U2OS using 80845-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MYC antibody (80845-1-RR, Clone: 2B17 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 80845-1-RR

    Affinity and Kinetic Characterization of 80845-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80845-1-RR against Human c-MYC were performed. The affinity constant is 2.03 nM.

Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, Hela cells, K-562 cells, HL-60 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HEK-293 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80845-1-RR targets c-MYC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen c-MYC fusion protein Ag1263
Full Name v-myc myelocytomatosis viral oncogene homolog (avian)
Calculated Molecular Weight 49 kDa
Observed Molecular Weight50-65 kDa
GenBank Accession NumberBC000141
Gene Symbol MYC
Gene ID (NCBI) 4609
RRIDAB_2918915
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.

Protocols

Product Specific Protocols
WB protocol for c-MYC antibody 80845-1-RRDownload protocol
IHC protocol for c-MYC antibody 80845-1-RRDownload protocol
IF protocol for c-MYC antibody 80845-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols