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P53 Recombinant antibody

Name: P53 Recombinant antibody
Brand: Proteintech
SKU: 80077-1-RR

P53 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

CloneNo.

2H13

Review Product

Cat no : 80077-1-RR

Synonyms

TP53, Tumour Protein p53, Tumor suppressor p53, tumor protein p53, Antigen NY-CO-13

Validation Data Gallery

Filter:

AllWBIHCIF/ICCFC (Intra)

WB analysis using 80077-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of zebrafish using 80077-1-RR
zebrafish tissue were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 80077-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 80077-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 80077-1-RR
WB result of P53 antibody (80077-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-P53 transfected A431 cells.
WB analysis using 80077-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 80077-1-RR
A431 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of SW480 using 80077-1-RR
SW480 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of HT-29 using 80077-1-RR
HT-29 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of ROS1728 using 80077-1-RR
ROS1728 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of HSC-T6 using 80077-1-RR
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of ATDC-5 using 80077-1-RR
ATDC-5 cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of Neuro-2a using 80077-1-RR
Neuro-2a cells were subjected to SDS PAGE followed by western blot with 80077-1-RR (P53 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 80077-1-RR
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80077-1-RR (P53 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 80077-1-RR
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using P53 antibody (80077-1-RR, Clone: 2H13 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of A431 using 80077-1-RR
Immunofluorescent analysis of (4% PFA) fixed A431 cells using P53 antibody (80077-1-RR, Clone: 2H13 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).
FC experiment of HepG2 using 80077-1-RR
1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human P53 (80077-1-RR, Clone:2H13) (red) labeled with FlexAble CoraLite®488 Antibody Labeling Kit for Rabbit IgG (KFA001), or 0.5 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Cat No. 80077-1-RR

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Tested Applications

Positive WB detected in	MCF-7 cells, COLO 320 cells, SW480 cells, MDA-MB-231 cells, HCT 116 cells, A431 cells, HT-29 cells, ROS1728 cells, HSC-T6 cells, ATDC-5 cells, Neuro-2a cells, zebrafish tissue, HT-29cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, A431 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below
IF	See 2 publications below

Product Information

80077-1-RR targets P53 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity	human, mouse, rat, zebrafish
Cited Reactivity	human, mouse, zebrafish
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide
Full Name	tumor protein p53
Calculated Molecular Weight	44 kDa
Observed Molecular Weight	53 kDa
GenBank Accession Number	BC003596
Gene Symbol	P53
Gene ID (NCBI)	7157
RRID	AB_2918862
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161)

Protocols

Product Specific Protocols
WB protocol for P53 antibody 80077-1-RR	Download protocol
IHC protocol for P53 antibody 80077-1-RR	Download protocol
IF protocol for P53 antibody 80077-1-RR	Download protocol
FC protocol for P53 antibody 80077-1-RR	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Oncol Rep Pseudolaric acid B induces apoptosis associated with the mitochondrial and PI3K/AKT/mTOR pathways in triple‑negative breast cancer Authors - Ke Yang View Article
mouse	WB,IF	Neurotox Res NRF2 Antagonizes HIV-1 Tat and Methamphetamine-Induced BV2 Cell Ferroptosis by Regulating SLC7A11 Authors - Shucheng Lin View Article
human	WB	Acta Biochim Biophys Sin (Shanghai) Hydrogen sulfide ameliorates senescence in vascular endothelial cells through ameliorating inflammation and activating PPARδ/SGLT2/STAT3 signaling pathway Authors - Danyang Tian View Article
zebrafish	WB	Cell Biol Toxicol Deficiency of copper responsive gene stmn4 induces retinal developmental defects Authors - YuanYuan Jing View Article
mouse	WB	Reprod Toxicol Cadmium disrupt spermatogenic cell cycle via piRNA-DQ717867/p53 pathway Authors - Jiaoyang Wei View Article
mouse	IF,WB	World J Gastroenterol Silent information regulator sirtuin 1 ameliorates acute liver failure via the p53/glutathione peroxidase 4/gasdermin D axis Authors - Xing-Nian Zhou View Article

P53 Recombinant antibody

P53 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Validation Data Gallery

WB analysis using 80077-1-RR

WB analysis of zebrafish using 80077-1-RR

WB analysis using 80077-1-RR

WB analysis using 80077-1-RR

WB analysis of A431 using 80077-1-RR

WB analysis using 80077-1-RR

WB analysis of A431 using 80077-1-RR

WB analysis of SW480 using 80077-1-RR

WB analysis of HT-29 using 80077-1-RR

WB analysis of ROS1728 using 80077-1-RR

WB analysis of HSC-T6 using 80077-1-RR

WB analysis of ATDC-5 using 80077-1-RR

WB analysis of Neuro-2a using 80077-1-RR

IHC staining of human colon cancer using 80077-1-RR

IF Staining of HepG2 using 80077-1-RR

IF Staining of A431 using 80077-1-RR

FC experiment of HepG2 using 80077-1-RR

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Pseudolaric acid B induces apoptosis associated with the mitochondrial and PI3K/AKT/mTOR pathways in triple‑negative breast cancer

NRF2 Antagonizes HIV-1 Tat and Methamphetamine-Induced BV2 Cell Ferroptosis by Regulating SLC7A11

Hydrogen sulfide ameliorates senescence in vascular endothelial cells through ameliorating inflammation and activating PPARδ/SGLT2/STAT3 signaling pathway

Deficiency of copper responsive gene stmn4 induces retinal developmental defects

Cadmium disrupt spermatogenic cell cycle via piRNA-DQ717867/p53 pathway

Silent information regulator sirtuin 1 ameliorates acute liver failure via the p53/glutathione peroxidase 4/gasdermin D axis

P53 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA