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  • Western Blot (WB) analysis of various lysates using DYNLT1 Monoclonal antibody (68312-1-Ig)

    WB analysis using 68312-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68312-1-Ig (DYNLT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using DYNLT1 Monoclonal antibody (68312-1-Ig)

    IF Staining of C2C12 using 68312-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using DYNLT1 antibody (68312-1-Ig, Clone: 2E3D4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of H9C2 cells using DYNLT1 Monoclonal antibody (68312-1-Ig)

    IF Staining of H9C2 using 68312-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using DYNLT1 antibody (68312-1-Ig, Clone: 2E3D4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

DYNLT1 Monoclonal antibody

DYNLT1 Monoclonal Antibody for WB, ELISA
Cat No. 68312-1-Ig
Clone No.2E3D4

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

CW 1, DYNLT1, Protein CW 1, TCTEL1, tctex 1, TCTEX1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68312-1-Ig targets DYNLT1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen DYNLT1 fusion protein Ag33072 Predict reactive species
Full Name dynein, light chain, Tctex-type 1
Calculated Molecular Weight 113 aa, 12 kDa
Observed Molecular Weight12 kDa
GenBank Accession NumberBC029412
Gene Symbol DYNLT1
Gene ID (NCBI) 6993
RRIDAB_2935390
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP63172
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DYNLT (or Tctex-1) was originally described as a light chain component of the dynein motor complex. Tctex-1 also has several dynein-independent functions, including roles in G protein signaling activation and neuronal growth. Tctex-1 is selectively enriched in proliferating neural progenitors of both embryonic and adult brains. Genetic knockdown of Tctex-1 in radial precursors promoted neurogenesis, indicating its implication in regulation of cortical neurogenesis. (PMID: 19448628)

Protocols

Product Specific Protocols
WB protocol for DYNLT1 antibody 68312-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols