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USP36 Monoclonal antibody
USP36 Monoclonal Antibody for WB, IP, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat, Mouse and More (1)
Applications
WB, IP, ELISA
Conjugate
Unconjugated
CloneNo.
1A1B3
Cat no : 68165-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | U2OS cells, K-562 cells, HeLa cells, A549 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IP detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
68165-1-Ig targets USP36 in WB, IP, ELISA applications and shows reactivity with Human, Rat, Mouse samples.
| Tested Reactivity | Human, Rat, Mouse |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | USP36 fusion protein Ag6426 |
| Full Name | ubiquitin specific peptidase 36 |
| Calculated Molecular Weight | 1123 aa, 123 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC038983 |
| Gene Symbol | USP36 |
| Gene ID (NCBI) | 57602 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
USP36 (Ubiquitin carboxyl-terminal hydrolase 36) is also named as KIAA1453, FLJ12851, DUB1 and belongs to the peptidase C19 family. It regulates the transcription and processing of rRNA by deubiquitylating various proteins involved in these processes, and the aberrant nucleolar structure in its absence is secondary to deficient ribosome biogenesis. USP36 plays a crucial role in regulating the structure and function of nucleoli (PMID:19208757). The ubiquitin-specific protease that is required in germline, epithelial, and intestinal stem cells (PMID:19039105). USP36 can be detected in 150 kDa (PMID:33852194)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for USP36 antibody 68165-1-Ig | Download protocol |
| IP protocol for USP36 antibody 68165-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Mol Sci CircMTA2 Drives Gastric Cancer Progression through Suppressing MTA2 Degradation via Interacting with UCHL3 |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Chrysa (Verified Customer) (02-01-2023) | Tested in HeLa cells in WB with control si and siRNA against USP36
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FH Chrysa (Verified Customer) (01-17-2023) | Unfortunately, due to couriers mistake the sample stayed 2 days at RT. I tried the antibody 3 times : 1/5000, 1:500 and 1/100 in 5%TBS-milk O/N at 4C in total lysates from HeLa cells (30μg) and didn't work (image uploaded, all the other antibodies worked in the same membrane e.g. anti-tubulin, anti-exosc10, anti-senp3). In 1/100 dilution I got non specific bands. Is it possible that the sample got degraded or is there anything I could do to optimize it to work?
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