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  • Featured Product
  • KD/KO Validated

USP36 Monoclonal antibody, PBS Only

USP36 Monoclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A1B3

Cat no : 68165-1-PBS

Synonyms

Deubiquitinating enzyme 36, DUB1, KIAA1453, Ubiquitin thiolesterase 36, USP36

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using USP36 Monoclonal antibody (68165-1-Ig)

    WB analysis using 68165-1-Ig (same clone as 68165-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68165-1-Ig (USP36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68165-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using USP36 Monoclonal antibody (68165-1-Ig)

    WB analysis using 68165-1-Ig (same clone as 68165-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68165-1-Ig (USP36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68165-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using USP36 Monoclonal antibody (68165-1-Ig)

    WB analysis of HeLa using 68165-1-Ig (same clone as 68165-1-PBS)

    WB result of USP36 antibody (68165-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP36 transfected HeLa cells. This data was developed using the same antibody clone with 68165-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using USP36 Monoclonal antibody (68165-1-Ig)

    IP experiment of Jurkat using 68165-1-Ig (same clone as 68165-1-PBS)

    IP result of anti-USP36 (IP:68165-1-Ig, 4ug; Detection:68165-1-Ig 1:3000) with Jurkat cells lysate 2030 ug. This data was developed using the same antibody clone with 68165-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68165-1-PBS targets USP36 in WB, IP, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen USP36 fusion protein Ag6426
Full Name ubiquitin specific peptidase 36
Calculated Molecular Weight 1123 aa, 123 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC038983
Gene Symbol USP36
Gene ID (NCBI) 57602
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

USP36 (Ubiquitin carboxyl-terminal hydrolase 36) is also named as KIAA1453, FLJ12851, DUB1 and belongs to the peptidase C19 family. It regulates the transcription and processing of rRNA by deubiquitylating various proteins involved in these processes, and the aberrant nucleolar structure in its absence is secondary to deficient ribosome biogenesis. USP36 plays a crucial role in regulating the structure and function of nucleoli (PMID:19208757). The ubiquitin-specific protease that is required in germline, epithelial, and intestinal stem cells (PMID:19039105). USP36 can be detected in 150 kDa (PMID:33852194)