USP36 Monoclonal antibody, PBS Only

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  WB analysis using 68165-1-Ig (same clone as 68165-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68165-1-Ig (USP36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68165-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68165-1-Ig (same clone as 68165-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68165-1-Ig (USP36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68165-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68165-1-Ig (same clone as 68165-1-PBS)

  WB result of USP36 antibody (68165-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP36 transfected HeLa cells. This data was developed using the same antibody clone with 68165-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 68165-1-Ig (same clone as 68165-1-PBS)

  IP result of anti-USP36 (IP:68165-1-Ig, 4ug; Detection:68165-1-Ig 1:3000) with Jurkat cells lysate 2030 ug. This data was developed using the same antibody clone with 68165-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68165-1-Ig (same clone as 68165-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP36 antibody (68165-1-Ig, Clone: 1A1B3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68165-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 68165-1-Ig (same clone as 68165-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug USP36 Monoclonal antibody (68165-1-Ig, Clone:1A1B3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68165-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

USP36 Monoclonal Antibody for WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Cat No. 68165-1-PBS

Clone No.1A1B3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

Ubiquitin carboxyl-terminal hydrolase 36, KIAA1453, EC:2.3.2.-, DUB1, Deubiquitinating enzyme 36

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Product Information

68165-1-PBS targets USP36 in WB, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: USP36 fusion protein Ag6426
• Full Name: ubiquitin specific peptidase 36
• Calculated Molecular Weight: 1123 aa, 123 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC038983
• Gene Symbol: USP36
• Gene ID (NCBI): 57602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9P275
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

USP36 (Ubiquitin carboxyl-terminal hydrolase 36) is also named as KIAA1453, FLJ12851, DUB1 and belongs to the peptidase C19 family. It regulates the transcription and processing of rRNA by deubiquitylating various proteins involved in these processes, and the aberrant nucleolar structure in its absence is secondary to deficient ribosome biogenesis. USP36 plays a crucial role in regulating the structure and function of nucleoli (PMID:19208757). The ubiquitin-specific protease that is required in germline, epithelial, and intestinal stem cells (PMID:19039105). USP36 can be detected in 150 kDa (PMID:33852194)