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  • Western Blot (WB) analysis of various lysates using SLC39A3 Monoclonal antibody (68025-1-Ig)

    WB analysis using 68025-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5200 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of HepG2 cells using SLC39A3 Monoclonal antibody (68025-1-Ig)

    WB analysis of HepG2 using 68025-1-Ig

    unboiled HepG2 cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using SLC39A3 Monoclonal antibody (68025-1-Ig)

    WB analysis of A549 using 68025-1-Ig

    37°C incubated A549 cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using SLC39A3 Monoclonal antibody (68025-1-Ig)

    WB analysis of LNCaP using 68025-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using SLC39A3 Monoclonal antibody (68025-1-Ig)

    WB analysis of Jurkat using 68025-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using SLC39A3 Monoclonal antibody (68025-1-Ig)

    WB analysis of K-562 using 68025-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

SLC39A3 Monoclonal antibody

SLC39A3 Monoclonal Antibody for WB, ELISA
Cat No. 68025-1-Ig
Clone No.1A11F6

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Synonyms

SLC39A3, Zinc transporter ZIP3, ZIP 3, ZIP3, Zrt and Irt like protein 3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inunboiled HepG2 cells, HepG2 cells, 37°C incubated A549 cells, LNCaP cells, Jurkat cells, K-562 cells, unboiled A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68025-1-Ig targets SLC39A3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SLC39A3 fusion protein Ag14289 Predict reactive species
Full Name solute carrier family 39 (zinc transporter), member 3
Calculated Molecular Weight 314 aa, 34 kDa
Observed Molecular Weight32 kDa
GenBank Accession NumberBC005869
Gene Symbol SLC39A3
Gene ID (NCBI) 29985
RRIDAB_2918769
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BRY0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC39A3, also named as ZIP3, belongs to the ZIP transporter (TC 2.A.5) family. SLC39A3 is a Zn importer that acts as a zinc-influx transporter. SLC39A3 is highly expressed in a cell-type-specific manner in ovaries, testes, and the developing nervous system with limited expression in the small intestinal crypt cells, kidney glomerulus, and discrete regions of the liver. SLC39A3 is also expressed in highly specialized, hormonally responsive secretory tissues, such as the pancreas, prostate, and mammary gland (PMID: 19458277). SLC39A3 has 2 isoforms with the molecular mass of 11 and 34 kDa.

Protocols

Product Specific Protocols
WB protocol for SLC39A3 antibody 68025-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols