SLC39A3 Monoclonal antibody, PBS Only

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  WB analysis using 68025-1-Ig (same clone as 68025-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5200 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68025-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68025-1-Ig (same clone as 68025-1-PBS)

  unboiled HepG2 cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68025-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 68025-1-Ig (same clone as 68025-1-PBS)

  37°C incubated A549 cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68025-1-PBS in a different storage buffer formulation.

• 

  WB analysis of LNCaP using 68025-1-Ig (same clone as 68025-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68025-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 68025-1-Ig (same clone as 68025-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68025-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 68025-1-Ig (same clone as 68025-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68025-1-PBS in a different storage buffer formulation.

SLC39A3 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 68025-1-PBS

Clone No.1A11F6

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

SLC39A3, Zinc transporter ZIP3, ZIP 3, ZIP3, Zrt and Irt like protein 3

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Product Information

68025-1-PBS targets SLC39A3 in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SLC39A3 fusion protein Ag14289
• Full Name: solute carrier family 39 (zinc transporter), member 3
• Calculated Molecular Weight: 314 aa, 34 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC005869
• Gene Symbol: SLC39A3
• Gene ID (NCBI): 29985
• RRID: AB_2918769
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BRY0
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

SLC39A3, also named as ZIP3, belongs to the ZIP transporter (TC 2.A.5) family. SLC39A3 is a Zn importer that acts as a zinc-influx transporter. SLC39A3 is highly expressed in a cell-type-specific manner in ovaries, testes, and the developing nervous system with limited expression in the small intestinal crypt cells, kidney glomerulus, and discrete regions of the liver. SLC39A3 is also expressed in highly specialized, hormonally responsive secretory tissues, such as the pancreas, prostate, and mammary gland (PMID: 19458277). SLC39A3 has 2 isoforms with the molecular mass of 11 and 34 kDa.