CoraLite® Plus 488-conjugated SLC39A3 Monoclonal antibody

• 

  FC experiment of A431 using CL488-68025

  1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SLC39A3 (CL488-68025, Clone:1A11F6) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

SLC39A3 Monoclonal Antibody for FC (Intra)

Cat No. CL488-68025

Clone No.1A11F6

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC (Intra)

Synonyms

Zrt- and Irt-like protein 3, Zrt and Irt like protein 3, ZIP-3, ZIP3, ZIP 3

---

Tested Applications

• Positive FC (Intra) detected in: A431 cells
• Positive FC detected in: A431 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68025 targets SLC39A3 in FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: SLC39A3 fusion protein Ag14289
• Full Name: solute carrier family 39 (zinc transporter), member 3
• Calculated Molecular Weight: 314 aa, 34 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC005869
• Gene Symbol: SLC39A3
• Gene ID (NCBI): 29985
• RRID: AB_2923863
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BRY0
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SLC39A3, also named as ZIP3, belongs to the ZIP transporter (TC 2.A.5) family. SLC39A3 is a Zn importer that acts as a zinc-influx transporter. SLC39A3 is highly expressed in a cell-type-specific manner in ovaries, testes, and the developing nervous system with limited expression in the small intestinal crypt cells, kidney glomerulus, and discrete regions of the liver. SLC39A3 is also expressed in highly specialized, hormonally responsive secretory tissues, such as the pancreas, prostate, and mammary gland (PMID: 19458277). SLC39A3 has 2 isoforms with the molecular mass of 11 and 34 kDa.