• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

AlaRS Monoclonal antibody

AlaRS Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C8H3

Cat no : 67909-1-Ig

Synonyms

AARS, AARS1, Alanine tRNA ligase, alanyl tRNA synthetase, AlaRS

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using AlaRS Monoclonal antibody (67909-1-Ig)

    WB analysis using 67909-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67909-1-Ig (AlaRS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • Western Blot (WB) analysis of HeLa cells using AlaRS Monoclonal antibody (67909-1-Ig)

    WB analysis of HeLa using 67909-1-Ig

    WB result of AlaRS antibody (67909-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AlaRS transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using AlaRS Monoclonal antibody (67909-1-Ig)

    WB analysis using 67909-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67909-1-Ig (AARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AlaRS Monoclonal antibody (67909-1-Ig)

    IF Staining of HepG2 using 67909-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AlaRS antibody (67909-1-Ig, Clone: 1C8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using AlaRS Monoclonal antibody (67909-1-Ig)

    IF Staining of A431 using 67909-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using AlaRS antibody (67909-1-Ig, Clone: 1C8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, 4T1 cells
Positive IF/ICC detected inHepG2 cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67909-1-Ig targets AlaRS in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AlaRS fusion protein Ag11151
Full Name alanyl-tRNA synthetase
Calculated Molecular Weight 968 aa, 107 kDa
Observed Molecular Weight107 kDa
GenBank Accession NumberBC011451
Gene Symbol AlaRS
Gene ID (NCBI) 16
RRIDAB_2918664
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AARS(alanyl-tRNA synthetase) is also named as AlaRS(alanine tRNA ligase 1, cytoplasmic), renal carcinoma antigen NY-REN-42 and belongs to the class-II aminoacyl-tRNA synthetase family. It can interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. AARS consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs(PMID:19661429).

Protocols

Product Specific Protocols
WB protocol for AlaRS antibody 67909-1-IgDownload protocol
IF protocol for AlaRS antibody 67909-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols