CoraLite®555-conjugated AlaRS Monoclonal antibody

• Featured Product
• KD/KO Validated

AlaRS Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1C8H3

Cat no : CL555-67909

Synonyms

AARS, 1C8H3, EC:6.1.1.7, Alanyl-tRNA synthetase, Alanine--tRNA ligase, cytoplasmic

---

Validation Data Gallery

• 

  IF Staining of HeLa using CL555-67909

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®555 AlaRS antibody (CL555-67909, Clone: 1C8H3 ) at dilution of 1:200.

• 

  FC experiment of K-562 using CL555-67909

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human AlaRS (CL555-67909, Clone:1C8H3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: K-562 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-67909 targets AlaRS in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AlaRS fusion protein Ag11151
• Full Name: alanyl-tRNA synthetase
• Calculated Molecular Weight: 968 aa, 107 kDa
• Observed Molecular Weight: 107 kDa
• GenBank Accession Number: BC011451
• Gene Symbol: AlaRS
• Gene ID (NCBI): 16
• RRID: AB_2919706
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

AARS(alanyl-tRNA synthetase) is also named as AlaRS(alanine tRNA ligase 1, cytoplasmic), renal carcinoma antigen NY-REN-42 and belongs to the class-II aminoacyl-tRNA synthetase family. It can interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. AARS consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs(PMID:19661429).

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C