AlaRS Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

AlaRS Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C8H3

Cat no : 67909-1-PBS

Synonyms

AARS, Alanine tRNA ligase, alanyl tRNA synthetase, AlaRS

---

Validation Data Gallery

• 

  WB analysis using 67909-1-Ig (same clone as 67909-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67909-1-Ig (AlaRS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 67909-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67909-1-Ig (same clone as 67909-1-PBS)

  WB result of AlaRS antibody (67909-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AlaRS transfected HeLa cells. This data was developed using the same antibody clone with 67909-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67909-1-Ig (same clone as 67909-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67909-1-Ig (AARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67909-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67909-1-Ig (same clone as 67909-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AlaRS antibody (67909-1-Ig, Clone: 1C8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67909-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 67909-1-Ig (same clone as 67909-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using AlaRS antibody (67909-1-Ig, Clone: 1C8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67909-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67909-1-PBS targets AlaRS in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: AlaRS fusion protein Ag11151
• Full Name: alanyl-tRNA synthetase
• Calculated Molecular Weight: 968 aa, 107 kDa
• Observed Molecular Weight: 107 kDa
• GenBank Accession Number: BC011451
• Gene Symbol: AlaRS
• Gene ID (NCBI): 16
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

AARS(alanyl-tRNA synthetase) is also named as AlaRS(alanine tRNA ligase 1, cytoplasmic), renal carcinoma antigen NY-REN-42 and belongs to the class-II aminoacyl-tRNA synthetase family. It can interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. AARS consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs(PMID:19661429).