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CIB1 Monoclonal antibody

CIB1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, bovine

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D2C12

Cat no : 67901-1-Ig

Synonyms

Calmyrin, CIB, CIB1, CIBP, DNA PKcs interacting protein, Kinase interacting protein, KIP, KIP1, PRKDCIP, SIP2 28, SNK interacting protein 2 28

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CIB1 Monoclonal antibody (67901-1-Ig)

    WB analysis using 67901-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67901-1-Ig (CIB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CIB1 Monoclonal antibody (67901-1-Ig)

    IHC staining of human breast cancer using 67901-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67901-1-Ig (CIB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CIB1 Monoclonal antibody (67901-1-Ig)

    IHC staining of human breast cancer using 67901-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67901-1-Ig (CIB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inLNCaP cells, Hela cells, K-562 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, Human milk
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67901-1-Ig targets CIB1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, bovine samples.

Tested Reactivity Human, mouse, rat, bovine
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CIB1 fusion protein Ag31174
Full Name calcium and integrin binding 1 (calmyrin)
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC000846
Gene Symbol CIB1
Gene ID (NCBI) 10519
RRIDAB_2918657
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CIB1 antibody 67901-1-IgDownload protocol
IHC protocol for CIB1 antibody 67901-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols