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CoraLite® Plus 488-conjugated CIB1 Monoclonal antibody

CIB1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, bovine

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D2C12

Cat no : CL488-67901

Synonyms

Calmyrin, CIB, CIB1, CIBP, DNA PKcs interacting protein, Kinase interacting protein, KIP, KIP1, PRKDCIP, SIP2 28, SNK interacting protein 2 28

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 488-conjugated CIB1 Monoclonal anti (CL488-67901)

    FC experiment of Jurkat using CL488-67901

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CIB1 (CL488-67901, Clone:1D2C12) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC (Intra) detected inJurkat cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67901 targets CIB1 in FC (Intra) applications and shows reactivity with Human, mouse, rat, bovine samples.

Tested Reactivity Human, mouse, rat, bovine
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CIB1 fusion protein Ag31174
Full Name calcium and integrin binding 1 (calmyrin)
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC000846
Gene Symbol CIB1
Gene ID (NCBI) 10519
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CIB1 antibody CL488-67901Download protocol
Standard Protocols
Click here to view our Standard Protocols