CIB1 Monoclonal antibody, PBS Only

CIB1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, bovine

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D2C12

Cat no : 67901-1-PBS

Synonyms

DNA PKcs interacting protein, CIBP, CIB, Calmyrin, Calcium and integrin-binding protein 1

---

Validation Data Gallery

• 

  WB analysis using 67901-1-Ig (same clone as 67901-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67901-1-Ig (CIB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67901-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67901-1-Ig (same clone as 67901-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67901-1-Ig (CIB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67901-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67901-1-Ig (same clone as 67901-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67901-1-Ig (CIB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67901-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 67901-1-Ig (same clone as 67901-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug CIB1 Monoclonal antibody (67901-1-Ig, Clone:1D2C12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67901-1-PBS in a different storage buffer formulation.

---

Product Information

67901-1-PBS targets CIB1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, bovine samples.

• Tested Reactivity: human, mouse, rat, bovine
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CIB1 fusion protein Ag31174
• Full Name: calcium and integrin binding 1 (calmyrin)
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC000846
• Gene Symbol: CIB1
• Gene ID (NCBI): 10519
• RRID: AB_2918657
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.