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DAXX Monoclonal antibody

DAXX Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F3G9

Cat no : 67879-1-Ig

Synonyms

BING2, DAP6, DAXX, EAP1, ETS1 associated protein 1, hDaxx

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using DAXX Monoclonal antibody (67879-1-Ig)

    WB analysis of Raji using 67879-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 67879-1-Ig (DAXX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Daudi cells using DAXX Monoclonal antibody (67879-1-Ig)

    WB analysis of Daudi using 67879-1-Ig

    Daudi cells were subjected to SDS PAGE followed by western blot with 67879-1-Ig (DAXX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MOLT-4 cells using DAXX Monoclonal antibody (67879-1-Ig)

    WB analysis of MOLT-4 using 67879-1-Ig

    MOLT-4 cells were subjected to SDS PAGE followed by western blot with 67879-1-Ig (DAXX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using DAXX Monoclonal antibody (67879-1-Ig)

    WB analysis of HeLa using 67879-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67879-1-Ig (DAXX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using DAXX Monoclonal antibody (67879-1-Ig)

    WB analysis of Jurkat using 67879-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67879-1-Ig (DAXX antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DAXX Monoclonal antibody (67879-1-Ig)

    IHC staining of human breast cancer using 67879-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67879-1-Ig (DAXX antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using DAXX Monoclonal antibody (67879-1-Ig)

    IHC staining of human breast cancer using 67879-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67879-1-Ig (DAXX antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DAXX Monoclonal antibody (67879-1-Ig)

    IF Staining of A431 using 67879-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DAXX antibody (67879-1-Ig, Clone: 1F3G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inRaji cells, HeLa cells, Daudi cells, MOLT-4 cells, Jurkat cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67879-1-Ig targets DAXX in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen DAXX fusion protein Ag14606
Full Name death-domain associated protein
Calculated Molecular Weight 740 aa, 81 kDa
Observed Molecular Weight100-130 kDa
GenBank Accession NumberBC109074
Gene Symbol DAXX
Gene ID (NCBI) 1616
RRIDAB_2918636
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Death domain-associated protein (DAXX) is a transcription repressor involved in both physiological and pathological conditions. DAXX overexpression is a common feature in diverse cancers, which correlates with tumorigenesis, disease progression and treatment resistance. Daxx migrated with an apparent molecular weight of approximately 100-130 kDa. (PMID: 31350900, PMID: 32203224, PMID: 31614769)

Protocols

Product Specific Protocols
WB protocol for DAXX antibody 67879-1-IgDownload protocol
IHC protocol for DAXX antibody 67879-1-IgDownload protocol
IF protocol for DAXX antibody 67879-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols