CoraLite® Plus 488-conjugated DAXX Monoclonal antibody

DAXX Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F3G9

Cat no : CL488-67879

Synonyms

BING2, DAP6, DAXX, EAP1, ETS1 associated protein 1, hDaxx

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Validation Data Gallery

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  IF Staining of A431 using CL488-67879

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 DAXX antibody (CL488-67879, Clone: 1F3G9 ) at dilution of 1:400.

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Tested Applications

• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67879 targets DAXX in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: DAXX fusion protein Ag14606
• Full Name: death-domain associated protein
• Calculated Molecular Weight: 740 aa, 81 kDa
• Observed Molecular Weight: 100-130 kDa
• GenBank Accession Number: BC109074
• Gene Symbol: DAXX
• Gene ID (NCBI): 1616
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Death domain-associated protein (DAXX) is a transcription repressor involved in both physiological and pathological conditions. DAXX overexpression is a common feature in diverse cancers, which correlates with tumorigenesis, disease progression and treatment resistance. Daxx migrated with an apparent molecular weight of approximately 100-130 kDa. (PMID: 31350900, PMID: 32203224, PMID: 31614769)