ANKRD1 Monoclonal antibody
ANKRD1 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, rat, rabbit, pig and More (1)
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1F11F1
Cat no : 67775-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse heart tissue, rabbit heart tissue, pig heart tissue |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 2 publications below |
Product Information
67775-1-Ig targets ANKRD1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.
| Tested Reactivity | Human, mouse, rat, rabbit, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | ANKRD1 fusion protein Ag29974 |
| Full Name | ankyrin repeat domain 1 (cardiac muscle) |
| Calculated Molecular Weight | 319 aa, 36 kDa |
| GenBank Accession Number | BC018667 |
| Gene Symbol | ANKRD1 |
| Gene ID (NCBI) | 27063 |
| RRID | AB_2918540 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ankyrin repeat domain-containing protein 1(ANKRD1) belongs to the conserved muscle ankyrin repeat protein(MARP) family, whose expression is induced in response to physiologic stress, injury and hypertrophy. Thus its expression mainly in activated vascular endothelial cells. ANKRD1 may regulate SMC proliferation through the CDKN1A pathway. It functions as a nuclear transcription repressor that regulate cardiac gene's expression
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ANKRD1 antibody 67775-1-Ig | Download protocol |
| IF protocol for ANKRD1 antibody 67775-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
