CoraLite® Plus 555-conjugated ANKRD1 Monoclonal antibody

ANKRD1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, rabbit, pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 555 Fluorescent Dye

CloneNo.

1F11F1

Cat no : CL555-67775

Synonyms

ALRP, ANKRD1, bA320F15.2, C 193, C193, Cardiac ankyrin repeat protein, CARP, CVARP, HA1A2, MCARP

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Validation Data Gallery

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  IF Staining of A431 using CL555-67775

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 555 ANKRD1 antibody (CL555-67775, Clone: 1F11F1 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-67775 targets ANKRD1 in IF/ICC applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

• Tested Reactivity: Human, mouse, rat, rabbit, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ANKRD1 fusion protein Ag29974
• Full Name: ankyrin repeat domain 1 (cardiac muscle)
• Calculated Molecular Weight: 319 aa, 36 kDa
• GenBank Accession Number: BC018667
• Gene Symbol: ANKRD1
• Gene ID (NCBI): 27063
• Conjugate: CoraLite® Plus 555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 554 nm / 570 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ankyrin repeat domain-containing protein 1(ANKRD1) belongs to the conserved muscle ankyrin repeat protein(MARP) family, whose expression is induced in response to physiologic stress, injury and hypertrophy. Thus its expression mainly in activated vascular endothelial cells. ANKRD1 may regulate SMC proliferation through the CDKN1A pathway. It functions as a nuclear transcription repressor that regulate cardiac gene's expression