ANKRD1 Monoclonal antibody, PBS Only

• 

  WB analysis of mouse heart using 67775-1-Ig (same clone as 67775-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 67775-1-Ig (ANKRD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67775-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit heart using 67775-1-Ig (same clone as 67775-1-PBS)

  rabbit heart tissue were subjected to SDS PAGE followed by western blot with 67775-1-Ig (ANKRD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67775-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig heart using 67775-1-Ig (same clone as 67775-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67775-1-Ig (ANKRD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67775-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 67775-1-Ig (same clone as 67775-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ANKRD1 antibody (67775-1-Ig, Clone: 1F11F1 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67775-1-PBS in a different storage buffer formulation.

ANKRD1 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 67775-1-PBS

Clone No.1F11F1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, ELISA

Synonyms

CVARP, CARP, C193, C 193, Ankyrin repeat domain-containing protein 1

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Product Information

67775-1-PBS targets ANKRD1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ANKRD1 fusion protein Ag29974
• Full Name: ankyrin repeat domain 1 (cardiac muscle)
• Calculated Molecular Weight: 319 aa, 36 kDa
• GenBank Accession Number: BC018667
• Gene Symbol: ANKRD1
• Gene ID (NCBI): 27063
• RRID: AB_2918540
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15327
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Ankyrin repeat domain-containing protein 1(ANKRD1) belongs to the conserved muscle ankyrin repeat protein(MARP) family, whose expression is induced in response to physiologic stress, injury and hypertrophy. Thus its expression mainly in activated vascular endothelial cells. ANKRD1 may regulate SMC proliferation through the CDKN1A pathway. It functions as a nuclear transcription repressor that regulate cardiac gene's expression