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IPO4 Monoclonal antibody

IPO4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F3H2

Cat no : 67549-1-Ig

Synonyms

FLJ23338, Imp4, Imp4b, importin 4, Importin 4b, IPO4, Ran binding protein 4, RanBP4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IPO4 Monoclonal antibody (67549-1-Ig)

    WB analysis using 67549-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67549-1-Ig (IPO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using IPO4 Monoclonal antibody (67549-1-Ig)

    IHC staining of human stomach cancer using 67549-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67549-1-Ig (IPO4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using IPO4 Monoclonal antibody (67549-1-Ig)

    IHC staining of human stomach cancer using 67549-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67549-1-Ig (IPO4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using IPO4 Monoclonal antibody (67549-1-Ig)

    IF Staining of MCF-7 using 67549-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using IPO4 antibody (67549-1-Ig, Clone: 2F3H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HL-60 cells, THP-1 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67549-1-Ig targets IPO4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IPO4 fusion protein Ag2281
Full Name importin 4
Calculated Molecular Weight 1081 aa, 118 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC003690
Gene Symbol IPO4
Gene ID (NCBI) 79711
RRIDAB_2882764
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Importin‐4 (IPO4) belongs to the importin β family which directs the cargo to pass through the nuclear pore complex via transient interaction with nucleoporins. IPO4 is responsible for transporting histones H3 and H4 into the nucleus for chromatin assembly. Recently IPO4 has been reported to contribute to gastric cancer progression and poor prognosis.

Protocols

Product Specific Protocols
WB protocol for IPO4 antibody 67549-1-IgDownload protocol
IHC protocol for IPO4 antibody 67549-1-IgDownload protocol
IF protocol for IPO4 antibody 67549-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols