• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

IPO4 Monoclonal antibody, PBS Only

IPO4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F3H2

Cat no : 67549-1-PBS

Synonyms

FLJ23338, Imp4, Imp4b, importin 4, Importin 4b, IPO4, Ran binding protein 4, RanBP4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using IPO4 Monoclonal antibody (67549-1-Ig)

    WB analysis using 67549-1-Ig (same clone as 67549-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67549-1-Ig (IPO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67549-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using IPO4 Monoclonal antibody (67549-1-Ig)

    IHC staining of human stomach cancer using 67549-1-Ig (same clone as 67549-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67549-1-Ig (IPO4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67549-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using IPO4 Monoclonal antibody (67549-1-Ig)

    IHC staining of human stomach cancer using 67549-1-Ig (same clone as 67549-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67549-1-Ig (IPO4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67549-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using IPO4 Monoclonal antibody (67549-1-Ig)

    IF Staining of MCF-7 using 67549-1-Ig (same clone as 67549-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using IPO4 antibody (67549-1-Ig, Clone: 2F3H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67549-1-PBS in a different storage buffer formulation.

Product Information

67549-1-PBS targets IPO4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IPO4 fusion protein Ag2281
Full Name importin 4
Calculated Molecular Weight 1081 aa, 118 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC003690
Gene Symbol IPO4
Gene ID (NCBI) 79711
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Importin‐4 (IPO4) belongs to the importin β family which directs the cargo to pass through the nuclear pore complex via transient interaction with nucleoporins. IPO4 is responsible for transporting histones H3 and H4 into the nucleus for chromatin assembly. Recently IPO4 has been reported to contribute to gastric cancer progression and poor prognosis.