CoraLite®594-conjugated IPO4 Monoclonal antibody

IPO4 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2F3H2

Cat no : CL594-67549

Synonyms

FLJ23338, Imp4, Imp4b, importin 4, Importin 4b, IPO4, Ran binding protein 4, RanBP4

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Validation Data Gallery

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  IF Staining of MCF-7 using CL594-67549

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CoraLite®594 IPO4 antibody (CL594-67549, Clone: 2F3H2 ) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67549 targets IPO4 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IPO4 fusion protein Ag2281
• Full Name: importin 4
• Calculated Molecular Weight: 1081 aa, 118 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC003690
• Gene Symbol: IPO4
• Gene ID (NCBI): 79711
• RRID: AB_2920156
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Importin‐4 (IPO4) belongs to the importin β family which directs the cargo to pass through the nuclear pore complex via transient interaction with nucleoporins. IPO4 is responsible for transporting histones H3 and H4 into the nucleus for chromatin assembly. Recently IPO4 has been reported to contribute to gastric cancer progression and poor prognosis.