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RHOC Monoclonal antibody

RHOC Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1G1C1

Cat no : 67542-1-Ig

Synonyms

ARHC, ARH9, Rho-related GTP-binding protein RhoC, Rho cDNA clone 9, H9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RHOC Monoclonal antibody (67542-1-Ig)

    WB analysis using 67542-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67542-1-Ig (RHOC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RHOC Monoclonal antibody (67542-1-Ig)

    WB analysis using 67542-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67542-1-Ig (RHOC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using RHOC Monoclonal antibody (67542-1-Ig)

    IHC staining of human small intestine using 67542-1-Ig

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67542-1-Ig (RHOC antibody) at dilution of 1:800 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RHOC Monoclonal antibody (67542-1-Ig)

    IF Staining of HeLa using 67542-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RHOC antibody (67542-1-Ig, Clone: 1G1C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using RHOC Monoclonal antibody (67542-1-Ig)

    FC experiment of HepG2 using 67542-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human RHOC (67542-1-Ig, Clone:1G1C1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, C6 cells, NIH/3T3 cells, HEK-293 cells, PC-12 cells, RAW 264.7 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67542-1-Ig targets RHOC in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RHOC fusion protein Ag14633
Full Name ras homolog gene family, member C
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC007245
Gene Symbol RHOC
Gene ID (NCBI) 389
RRIDAB_2882761
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ras homolog family member C (RHOC) encodes a Rho-related GTP-binding protein, which belongs to small GTPase superfamily and also Rho family. RHOC. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Expression of RHOC contributes to metastatic phenotype of melanoma cells and is associated with multiple cancers including gastric carcinomas, ovarian carcinomas and breast cancer as well.

Protocols

Product Specific Protocols
WB protocol for RHOC antibody 67542-1-IgDownload protocol
IHC protocol for RHOC antibody 67542-1-IgDownload protocol
IF protocol for RHOC antibody 67542-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols