CoraLite® Plus 488-conjugated RHOC Monoclonal antibody

RHOC Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G1C1

Cat no : CL488-67542

Synonyms

ARH9, ARHC, H9, Rho cDNA clone 9, RHOC, RHOH9

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Validation Data Gallery

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  IF Staining of HeLa using CL488-67542

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 RHOC antibody (CL488-67542, Clone: 1G1C1 ) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67542 targets RHOC in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RHOC fusion protein Ag14633
• Full Name: ras homolog gene family, member C
• Calculated Molecular Weight: 22 kDa
• GenBank Accession Number: BC007245
• Gene Symbol: RHOC
• Gene ID (NCBI): 389
• RRID: AB_2919497
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ras homolog family member C (RHOC) encodes a Rho-related GTP-binding protein, which belongs to small GTPase superfamily and also Rho family. RHOC. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Expression of RHOC contributes to metastatic phenotype of melanoma cells and is associated with multiple cancers including gastric carcinomas, ovarian carcinomas and breast cancer as well.