RHOC Monoclonal antibody, PBS Only

RHOC Monoclonal Antibody for WB, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G1C1

Cat no : 67542-1-PBS

Synonyms

ARH9, ARHC, H9, Rho cDNA clone 9, RHOC, RHOH9

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Validation Data Gallery

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  WB analysis using 67542-1-Ig (same clone as 67542-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67542-1-Ig (RHOC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67542-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67542-1-Ig (same clone as 67542-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67542-1-Ig (RHOC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67542-1-PBS in a different storage buffer formulation.

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  IHC staining of human small intestine using 67542-1-Ig (same clone as 67542-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67542-1-Ig (RHOC antibody) at dilution of 1:800 (under 40x lens). This data was developed using the same antibody clone with 67542-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67542-1-Ig (same clone as 67542-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RHOC antibody (67542-1-Ig, Clone: 1G1C1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67542-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 67542-1-Ig (same clone as 67542-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human RHOC (67542-1-Ig, Clone:1G1C1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67542-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67542-1-PBS targets RHOC in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RHOC fusion protein Ag14633
• Full Name: ras homolog gene family, member C
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC007245
• Gene Symbol: RHOC
• Gene ID (NCBI): 389
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Ras homolog family member C (RHOC) encodes a Rho-related GTP-binding protein, which belongs to small GTPase superfamily and also Rho family. RHOC. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Expression of RHOC contributes to metastatic phenotype of melanoma cells and is associated with multiple cancers including gastric carcinomas, ovarian carcinomas and breast cancer as well.