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SEC63 Monoclonal antibody

SEC63 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C10E1

Cat no : 67352-1-Ig

Synonyms

ERdj2, PRO2507, SEC63, SEC63 homolog (S. cerevisiae), SEC63L

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SEC63 Monoclonal antibody (67352-1-Ig)

    WB analysis using 67352-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67352-1-Ig (SEC63 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SEC63 Monoclonal antibody (67352-1-Ig)

    IF Staining of HEK-293 using 67352-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SEC63 antibody (67352-1-Ig, Clone: 1C10E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67352-1-Ig targets SEC63 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SEC63 fusion protein Ag5783
Full Name SEC63 homolog (S. cerevisiae)
Calculated Molecular Weight 88 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC047221
Gene Symbol SEC63
Gene ID (NCBI) 11231
RRIDAB_2882608
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEC63 is a integral endoplasmic reticulum (ER) membrane protein. SEC63 and SEC62 form a dimeric complex and play a central role in translocation of nascent and newly synthesized precursor polypeptides into the ER. Mutations in the gene encoding SEC63 cause polycystic liver disease in humans. The intact SEC63 protein is usually detected around 83-90 kDa, while smaller cleavage products can be observed by autophagy proteolysis (PMID: 25052096)

Protocols

Product Specific Protocols
WB protocol for SEC63 antibody 67352-1-IgDownload protocol
IF protocol for SEC63 antibody 67352-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols