SEC63 Monoclonal antibody, PBS Only

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  WB analysis using 67352-1-Ig (same clone as 67352-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67352-1-Ig (SEC63 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67352-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 67352-1-Ig (same clone as 67352-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SEC63 antibody (67352-1-Ig, Clone: 1C10E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67352-1-PBS in a different storage buffer formulation.

SEC63 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 67352-1-PBS

Clone No.1C10E1

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

ERdj2, PRO2507, SEC63, SEC63 homolog (S. cerevisiae), SEC63L

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Product Information

67352-1-PBS targets SEC63 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SEC63 fusion protein Ag5783
• Full Name: SEC63 homolog (S. cerevisiae)
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC047221
• Gene Symbol: SEC63
• Gene ID (NCBI): 11231
• RRID: AB_2882608
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9UGP8
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

SEC63 is a integral ER membrane protein. SEC63 and SEC62 form a dimeric complex and play a central role in translocation of nascent and newly synthesized precursor polypeptides into the ER. Mutations in the gene encoding SEC63 cause polycystic liver disease in humans. The intact SEC63 protein is usually detected around 83-90 kDa, while smaller cleavage products can be observed by autophagy proteolysis (PMID: 25052096)