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VAMP4 Monoclonal antibody

VAMP4 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Rat, Mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3A6F2

Cat no : 67219-1-Ig

Synonyms

VAMP 4, VAMP24, VAMP4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using VAMP4 Monoclonal antibody (67219-1-Ig)

    WB analysis using 67219-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67219-1-Ig (VAMP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VAMP4 Monoclonal antibody (67219-1-Ig)

    IHC staining of mouse brain using 67219-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VAMP4 Monoclonal antibody (67219-1-Ig)

    IHC staining of mouse brain using 67219-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using VAMP4 Monoclonal antibody (67219-1-Ig)

    IF Staining of HEK-293 using 67219-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 67219-1-Ig (VAMP4 antibody), at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, K-562 cells, pig brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

67219-1-Ig targets VAMP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Pig, Rat, Mouse samples.

Tested Reactivity Human, Pig, Rat, Mouse
Cited Reactivitymouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen VAMP4 fusion protein Ag27854
Full Name vesicle-associated membrane protein 4
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC007019
Gene Symbol VAMP4
Gene ID (NCBI) 8674
RRIDAB_2882510
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VAMP4 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP4 may play a role in trans-Golgi network to endosome transport.

Protocols

Product Specific Protocols
WB protocol for VAMP4 antibody 67219-1-IgDownload protocol
IHC protocol for VAMP4 antibody 67219-1-IgDownload protocol
IF protocol for VAMP4 antibody 67219-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

eNeuro

ADP ribosylation factor 4 (Arf4) regulates radial migration through N-cadherin trafficking during cerebral cortical development

Authors - Yoshinobu Hara
View Article