CoraLite®594-conjugated VAMP4 Monoclonal antibody

VAMP4 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Rat, Mouse

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3A6F2

Cat no : CL594-67219

Synonyms

VAMP 4, VAMP24, VAMP4

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Validation Data Gallery

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  IF Staining of HEK-293 using CL594-67219

  Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using CoraLite®594 VAMP4 antibody (CL594-67219, Clone: 3A6F2 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67219 targets VAMP4 in IF/ICC applications and shows reactivity with Human, Pig, Rat, Mouse samples.

• Tested Reactivity: Human, Pig, Rat, Mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: VAMP4 fusion protein Ag27854
• Full Name: vesicle-associated membrane protein 4
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC007019
• Gene Symbol: VAMP4
• Gene ID (NCBI): 8674
• RRID: AB_2920099
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

VAMP4 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP4 may play a role in trans-Golgi network to endosome transport.