CoraLite® Plus 488-conjugated FSHB Monoclonal antibody

FSHB Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B10A11

Cat no : CL488-67131

Synonyms

FSH beta, FSH B, Follitropin subunit beta, Follitropin beta chain, Follicle-stimulating hormone beta subunit

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-67131

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 FSHB antibody (CL488-67131, Clone: 1B10A11 ) at dilution of 1:200.

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  IF Staining of HepG2 using CL488-67131

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 FSHB antibody (CL488-67131, Clone: 1B10A11 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67131 targets FSHB in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: FSHB fusion protein Ag13788
• Full Name: follicle stimulating hormone, beta polypeptide
• Calculated Molecular Weight: 129 aa, 15 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC113488
• Gene Symbol: FSHB
• Gene ID (NCBI): 2488
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The FSHB gene encodes the beta subunit of follicle-stimulating hormone (FSH), particularly expressed in gonadotroph cells within the anterior pituitary gland and plays a critical role in the regulation of gonadal function, pubertal maturation and reproductive processes in mammals. The transcription of the FSHB gene is critical for the production of the FSH hormone(PMID:28281143). And FSH is required for ovarian folliculogenesis in females, in males it promotes spermatogenesis in conjunction with testosterone(PMID:11739331). Mutations in FSHB gene would result in infertility both in men and women (PMID: 23766128).