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TFAP2A/AP-2 Monoclonal antibody

TFAP2A/AP-2 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1B12F11

Cat no : 67076-1-Ig

Synonyms

TFAP2A, TFAP2A,AP-2, AP-2, AP2, AP 2alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using TFAP2A/AP-2 Monoclonal antibody (67076-1-Ig)

    WB analysis of L02 using 67076-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using TFAP2A/AP-2 Monoclonal antibody (67076-1-Ig)

    WB analysis of HepG2 using 67076-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TFAP2A/AP-2 Monoclonal antibody (67076-1-Ig)

    WB analysis of MCF-7 using 67076-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using TFAP2A/AP-2 Monoclonal antibody (67076-1-Ig)

    WB analysis of HSC-T6 using 67076-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TFAP2A/AP-2 Monoclonal antibody (67076-1-Ig)

    WB analysis of HeLa using 67076-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TFAP2A/AP-2 Monoclonal antibody (67076-1-Ig)

    IHC staining of human breast cancer using 67076-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TFAP2A/AP-2 Monoclonal antibody (67076-1-Ig)

    IHC staining of human breast cancer using 67076-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67076-1-Ig (TFAP2A/AP-2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of A431 cells using TFAP2A/AP-2 Monoclonal antibody (67076-1-Ig)

    FC experiment of A431 using 67076-1-Ig

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human TFAP2A/AP-2 (67076-1-Ig, Clone:1B12F11) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inL02 cells, HeLa cells, HSC-T6 cells, MCF-7 cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

67076-1-Ig targets TFAP2A/AP-2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TFAP2A/AP-2 fusion protein Ag4112
Full Name transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)
Calculated Molecular Weight 431 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC017754
Gene Symbol TFAP2A/AP-2
Gene ID (NCBI) 7020
RRIDAB_2882384
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The activator protein-2 (AP-2) family of transcription factors comprises five 52-kDa isoforms (AP-2α, AP-2β, AP-2γ, AP-2δ, and AP-2ε), which share a common structure: a proline/glutamine-rich transactivation domain in the N-terminal region and a helix-span-helix domain in the C-terminal region, which mediates dimerization and site-specific DNA binding. Depending on the cellular context, the AP-2 transcription factors are individually associated either with cell differentiation and development or with cancer progression/regression. [PMID:21966377] TFAP2A (AP-2-alpha) is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation [PMID:11694877]

Protocols

Product Specific Protocols
WB protocol for TFAP2A/AP-2 antibody 67076-1-IgDownload protocol
IHC protocol for TFAP2A/AP-2 antibody 67076-1-IgDownload protocol
FC protocol for TFAP2A/AP-2 antibody 67076-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Genet

Identifification and validation of ferroptosis signatures and immune infifiltration characteristics associated with intervertebral disc degeneration

Authors - Feng Zhang
View Article
humanIHC

Oncol Rep

TFAP2A promotes cervical cancer via a positive feedback pathway with PD‑L1

Authors - Junyuan Yang
View Article