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CTBP1 Monoclonal antibody

CTBP1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1C4B2

Cat no : 66995-1-Ig

Synonyms

EC:1.1.1.-, C-terminal-binding protein 1, CTBP, BARS, 1C4B2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CTBP1 Monoclonal antibody (66995-1-Ig)

    WB analysis using 66995-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CTBP1 Monoclonal antibody (66995-1-Ig)

    WB analysis using 66995-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HEK-293 cells using CTBP1 Monoclonal antibody (66995-1-Ig)

    WB analysis of HEK-293 using 66995-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using CTBP1 Monoclonal antibody (66995-1-Ig)

    WB analysis of A549 using 66995-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HL-60 cells using CTBP1 Monoclonal antibody (66995-1-Ig)

    WB analysis of HL-60 using 66995-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using CTBP1 Monoclonal antibody (66995-1-Ig)

    WB analysis of HSC-T6 using 66995-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66995-1-Ig (CTBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CTBP1 Monoclonal antibody (66995-1-Ig)

    IHC staining of human breast cancer using 66995-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CTBP1 Monoclonal antibody (66995-1-Ig)

    IHC staining of human prostate cancer using 66995-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CTBP1 Monoclonal antibody (66995-1-Ig)

    IHC staining of human prostate cancer using 66995-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CTBP1 Monoclonal antibody (66995-1-Ig)

    IHC staining of human breast cancer using 66995-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66995-1-Ig (CTBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CTBP1 Monoclonal antibody (66995-1-Ig)

    IF Staining of HepG2 using 66995-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66995-1-Ig (CTBP1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CTBP1 Monoclonal antibody (66995-1-Ig)

    IF Staining of HepG2 using 66995-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CTBP1 antibody (66995-1-Ig, Clone: 1C4B2 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using CTBP1 Monoclonal antibody (66995-1-Ig)

    FC experiment of HepG2 using 66995-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human CTBP1 (66995-1-Ig, Clone:1C4B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells, A549 cells, HL-60 cells, HSC-T6 cells, HeLa cells, NCCIT cells, LNCaP cells, Jurkat cells, 4T1 cells, RAW 264.7 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:2000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66995-1-Ig targets CTBP1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CTBP1 fusion protein Ag28567
Full Name C-terminal binding protein 1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 45-48 kDa
GenBank Accession NumberBC011655
Gene Symbol CTBP1
Gene ID (NCBI) 1487
RRIDAB_2882312
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTBP1, also named as C-terminal-binding protein 1, is a 440 amino acid protein, which belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. CTBP1 is a cellular phosphoprotein that associates with various proteins and functions as a corepressor of transcription. CTBP1 and the related protein CTBP2 are characterized as C-terminal binding protein of adenovirus E1A, and they preferentially associate with the E1A via a 5-amino acid motif, PLDLS, to repress E1A induced oncogenesis and cellular transformation. CTBP1 is expressed from embryo to adult, but CTBP2 is mainly expressed during embryogenesis.

Protocols

Product Specific Protocols
WB protocol for CTBP1 antibody 66995-1-IgDownload protocol
IHC protocol for CTBP1 antibody 66995-1-IgDownload protocol
IF protocol for CTBP1 antibody 66995-1-IgDownload protocol
FC protocol for CTBP1 antibody 66995-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols