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  • Featured Product
  • KD/KO Validated

FOX2/RBM9 Monoclonal antibody

FOX2/RBM9 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D7G5

Cat no : 66976-1-Ig

Synonyms

dJ106I20.3, Fox 1 homolog B, Fox 2, FOX2, FOX2/RBM9, fxh, HNRBP2, HRNBP2, RBFOX2, RBM9, RNA binding motif protein 9, RNA binding protein 9, RTA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    WB analysis using 66976-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HEK-293 cells using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    WB analysis of HEK-293 using 66976-1-Ig

    WB result of RBM9 antibody (66976-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM9 transfected HEK-293 cells.

  • Western Blot (WB) analysis of HT-29 cells using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    WB analysis of HT-29 using 66976-1-Ig

    HT-29 cells were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NCCIT cells using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    WB analysis of NCCIT using 66976-1-Ig

    NCCIT cell were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    WB analysis of fetal human brain using 66976-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    WB analysis of human placenta using 66976-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    IHC staining of mouse brain using 66976-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    IHC staining of human pancreas cancer using 66976-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66976-1-Ig (FOX2/RBM9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    IHC staining of human pancreas cancer using 66976-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66976-1-Ig (FOX2/RBM9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    IHC staining of mouse brain using 66976-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    IHC staining of human ovary tumor using 66976-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    IHC staining of human ovary tumor using 66976-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    IHC staining of human lung cancer using 66976-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FOX2/RBM9 Monoclonal antibody (66976-1-Ig)

    IHC staining of human lung cancer using 66976-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HT-29 cells, NCCIT cells, human placenta tissue, fetal human brain tissue, HEK-293 cells, HepG2 cells, L02 cells
Positive IHC detected inmouse brain tissue, human ovary tumor tissue, human lung cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66976-1-Ig targets FOX2/RBM9 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FOX2/RBM9 fusion protein Ag28336
Full Name RNA binding motif protein 9
Calculated Molecular Weight 390 aa, 41 kDa
Observed Molecular Weight 48 kDa, 50 and 60 kDa
GenBank Accession NumberBC013115
Gene Symbol RBM9
Gene ID (NCBI) 23543
RRIDAB_2882296
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)

Protocols

Product Specific Protocols
WB protocol for FOX2/RBM9 antibody 66976-1-IgDownload protocol
IHC protocol for FOX2/RBM9 antibody 66976-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols