CoraLite® Plus 488-conjugated FOX2/RBM9 Monoclonal antibody

• Featured Product
• KD/KO Validated

FOX2/RBM9 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D7G5

Cat no : CL488-66976

Synonyms

dJ106I20.3, Fox 1 homolog B, Fox 2, FOX2, fxh, HNRBP2, HRNBP2, RBM9, RNA binding motif protein 9, RNA binding protein 9, RTA

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-66976

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 FOX2/RBM9 antibody (CL488-66976, Clone: 2D7G5 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66976 targets FOX2/RBM9 in IF/ICC applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FOX2/RBM9 fusion protein Ag28336
• Full Name: RNA binding motif protein 9
• Calculated Molecular Weight: 390 aa, 41 kDa
• Observed Molecular Weight: 48 kDa, 50 and 60 kDa
• GenBank Accession Number: BC013115
• Gene Symbol: RBM9
• Gene ID (NCBI): 23543
• RRID: AB_2919399
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)