FOX2/RBM9 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

FOX2/RBM9 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D7G5

Cat no : 66976-1-PBS

Synonyms

dJ106I20.3, Fox 1 homolog B, Fox 2, FOX2, FOX2/RBM9, fxh, HNRBP2, HRNBP2, RBFOX2, RBM9, RNA binding motif protein 9, RNA binding protein 9, RTA

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Validation Data Gallery

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  WB analysis using 66976-1-Ig (same clone as 66976-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66976-1-Ig (same clone as 66976-1-PBS)

  WB result of RBM9 antibody (66976-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM9 transfected HEK-293 cells. This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

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  WB analysis of human placenta using 66976-1-Ig (same clone as 66976-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 66976-1-Ig (same clone as 66976-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 66976-1-Ig (same clone as 66976-1-PBS)

  NCCIT cell were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66976-1-Ig (same clone as 66976-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66976-1-Ig (same clone as 66976-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66976-1-Ig (same clone as 66976-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66976-1-Ig (FOX2/RBM9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 66976-1-Ig (same clone as 66976-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66976-1-Ig (FOX2/RBM9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66976-1-Ig (same clone as 66976-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66976-1-Ig (same clone as 66976-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66976-1-Ig (same clone as 66976-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66976-1-Ig (same clone as 66976-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66976-1-Ig (same clone as 66976-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

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Product Information

66976-1-PBS targets FOX2/RBM9 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FOX2/RBM9 fusion protein Ag28336
• Full Name: RNA binding motif protein 9
• Calculated Molecular Weight: 390 aa, 41 kDa
• Observed Molecular Weight: 48 kDa, 50 and 60 kDa
• GenBank Accession Number: BC013115
• Gene Symbol: RBM9
• Gene ID (NCBI): 23543
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)