S6 Ribosomal protein Monoclonal antibody
S6 Ribosomal protein Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
1C3E10
Cat no : 66886-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A431 cells, NIH/3T3 cells, rat liver tissue, HEEK-293 cells, Jurkat cells, HSC-T6 cells, RAW 264.7 cells, HEK-293 cells |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 11 publications below |
| IF | See 1 publications below |
| FC | See 1 publications below |
Product Information
66886-1-Ig targets S6 Ribosomal protein in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | S6 Ribosomal protein fusion protein Ag6599 |
| Full Name | ribosomal protein S6 |
| Calculated Molecular Weight | 29 kDa |
| Observed Molecular Weight | 29-32 kDa |
| GenBank Accession Number | BC000524 |
| Gene Symbol | RPS6 |
| Gene ID (NCBI) | 6194 |
| RRID | AB_2882218 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ribosomal protein S6 (RPS6),Phosphoprotein NP33.It may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA.Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. Phosphorylated at Ser-235 and Ser-236 by RPS6KA1 and RPS6KA3; phosphorylation at these sites facilitates the assembly of the preinitiation complex.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for S6 Ribosomal protein antibody 66886-1-Ig | Download protocol |
| IF protocol for S6 Ribosomal protein antibody 66886-1-Ig | Download protocol |
| FC protocol for S6 Ribosomal protein antibody 66886-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Genome Biol A post-transcriptional program of chemoresistance by AU-rich elements and TTP in quiescent leukemic cells. | ||
Transl Res Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication. | ||
Microbiol Spectr Structural Basis and Function of the N Terminus of SARS-CoV-2 Nonstructural Protein 1. | ||
Ecotoxicol Environ Saf Exposure to DEHP induces testis toxicity and injury through the ROS/mTOR/NLRP3 signaling pathway in immature rats. | ||
Sci Rep YB1 associates with oncogenetic roles and poor prognosis in nasopharyngeal carcinoma. | ||
Endocrinology Wnt5a Regulates Junctional Function of Sertoli cells Through PCP-Mediated Effects on mTORC1 and mTORC2. |
