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  • Featured Product
  • KD/KO Validated

SERPING1/C1 Inactivator Monoclonal antibody

SERPING1/C1 Inactivator Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig, rat and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B11B11

Cat no : 66882-1-Ig

Synonyms

C1 esterase inhibitor, C1 Inh, C1 inhibiting factor, C1IN, C1INH, C1NH, HAE1, HAE2, Plasma protease C1 inhibitor, Serpin G1, SERPING1, SERPING1/C1 Inactivator

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SERPING1/C1 Inactivator Monoclonal antibody (66882-1-Ig)

    WB analysis using 66882-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66882-1-Ig (SERPING1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SERPING1/C1 Inactivator Monoclonal antibody (66882-1-Ig)

    IF Staining of HeLa using 66882-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SERPING1/C1 Inactivator antibody (66882-1-Ig, Clone: 1B11B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SERPING1/C1 Inactivator Monoclonal antibody (66882-1-Ig)

    IF Staining of HepG2 using 66882-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SERPING1/C1 Inactivator antibody (66882-1-Ig, Clone: 1B11B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SERPING1/C1 Inactivator Monoclonal antibody (66882-1-Ig)

    IF Staining of HepG2 using 66882-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SERPING1/C1 Inactivator antibody (66882-1-Ig, Clone: 1B11B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman plasma tissue, human plasma, pig plasma, rat serum
Positive IF/ICC detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66882-1-Ig targets SERPING1/C1 Inactivator in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig, rat samples.

Tested Reactivity Human, pig, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen SERPING1/C1 Inactivator fusion protein Ag16782
Full Name serpin peptidase inhibitor, clade G (C1 inhibitor), member 1
Calculated Molecular Weight 500 aa, 55 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC011171
Gene Symbol SERPING1
Gene ID (NCBI) 710
RRIDAB_2882214
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPING1, also known as C1 Inhibitor (C1INH), is a member of the Serine proteinase inhibitor family. Its main function is the inhibition of the complement system to prevent spontaneous activation. It inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE). C1INH is the most heavily glycosylated plasma protein.

Protocols

Product Specific Protocols
WB protocol for SERPING1/C1 Inactivator antibody 66882-1-IgDownload protocol
IF protocol for SERPING1/C1 Inactivator antibody 66882-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

Targeting endometrial inflammation in intrauterine adhesion ameliorates endometrial fibrosis by priming MSCs to secrete C1INH

Authors - Simin Yao
  • KD Validated
View Article